diff --git a/pkgs/development/python-modules/cryptography/default.nix b/pkgs/development/python-modules/cryptography/default.nix
index 8346b4f4418d..9c6aef0b394e 100644
--- a/pkgs/development/python-modules/cryptography/default.nix
+++ b/pkgs/development/python-modules/cryptography/default.nix
@@ -3,25 +3,25 @@
 , callPackage
 , buildPythonPackage
 , fetchPypi
-, rustPlatform
 , cargo
-, rustc
-, setuptoolsRustBuildHook
-, openssl
-, Security
-, isPyPy
 , cffi
+, hypothesis
+, iso8601
+, isPyPy
+, libiconv
+, libxcrypt
+, openssl
 , pkg-config
+, pretend
+, py
 , pytestCheckHook
 , pytest-subtests
 , pythonOlder
-, pretend
-, libiconv
-, libxcrypt
-, iso8601
-, py
 , pytz
-, hypothesis
+, rustc
+, rustPlatform
+, Security
+, setuptoolsRustBuildHook
 }:
 
 let
@@ -29,20 +29,20 @@ let
 in
 buildPythonPackage rec {
   pname = "cryptography";
-  version = "40.0.1"; # Also update the hash in vectors.nix
-  format = "setuptools";
-  disabled = pythonOlder "3.6";
+  version = "41.0.1"; # Also update the hash in vectors.nix
+  format = "pyproject";
+  disabled = pythonOlder "3.7";
 
   src = fetchPypi {
     inherit pname version;
-    hash = "sha256-KAPy+LHpX2FEGZJsfm9V2CivxhTKXtYVQ4d65mjMNHI=";
+    hash = "sha256-00V5CFQB0/SXYtL31mNNa2wq4SQiAuhg9NJrBG46EAY=";
   };
 
   cargoDeps = rustPlatform.fetchCargoTarball {
     inherit src;
     sourceRoot = "${pname}-${version}/${cargoRoot}";
     name = "${pname}-${version}";
-    hash = "sha256-gFfDTc2QWBWHBCycVH1dYlCsWQMVcRZfOBIau+njtDU=";
+    hash = "sha256-38q81vRf8QHR8lFRM2KbH7Ng5nY7nmtWRMoPWS9VO/U=";
   };
 
   postPatch = ''
@@ -100,8 +100,6 @@ buildPythonPackage rec {
       Cryptography includes both high level recipes and low level interfaces to
       common cryptographic algorithms such as symmetric ciphers, message
       digests, and key derivation functions.
-      Our goal is for it to be your "cryptographic standard library". It
-      supports Python 2.7, Python 3.5+, and PyPy 5.4+.
     '';
     homepage = "https://github.com/pyca/cryptography";
     changelog = "https://cryptography.io/en/latest/changelog/#v"
diff --git a/pkgs/development/python-modules/cryptography/vectors.nix b/pkgs/development/python-modules/cryptography/vectors.nix
index 71bd22bf738f..a87b049874ed 100644
--- a/pkgs/development/python-modules/cryptography/vectors.nix
+++ b/pkgs/development/python-modules/cryptography/vectors.nix
@@ -1,17 +1,19 @@
-{ buildPythonPackage, fetchPypi, lib, cryptography }:
+{ buildPythonPackage, fetchPypi, lib, cryptography, setuptools }:
 
 buildPythonPackage rec {
   pname = "cryptography-vectors";
   # The test vectors must have the same version as the cryptography package
   inherit (cryptography) version;
-  format = "setuptools";
+  format = "pyproject";
 
   src = fetchPypi {
     pname = "cryptography_vectors";
     inherit version;
-    hash = "sha256-hGBwa1tdDOSoVXHKM4nPiPcAu2oMYTPcn+D1ovW9oEE=";
+    hash = "sha256-I7CMOXd+x7J3ShH5RdF0YwGx6I7P8uUybX9Q6g9C1YA=";
   };
 
+  nativeBuildInputs = [ setuptools ];
+
   # No tests included
   doCheck = false;
 
@@ -20,7 +22,7 @@ buildPythonPackage rec {
   meta = with lib; {
     description = "Test vectors for the cryptography package";
     homepage = "https://cryptography.io/en/latest/development/test-vectors/";
-    # Source: https://github.com/pyca/cryptography/tree/master/vectors;
+    downloadPage = "https://github.com/pyca/cryptography/tree/master/vectors";
     license = with licenses; [ asl20 bsd3 ];
     maintainers = with maintainers; [ SuperSandro2000 ];
   };