diff --git a/nixos/tests/all-tests.nix b/nixos/tests/all-tests.nix
index f8e0eb190bf5..f548a02551af 100644
--- a/nixos/tests/all-tests.nix
+++ b/nixos/tests/all-tests.nix
@@ -1055,6 +1055,7 @@ in {
   unbound = handleTest ./unbound.nix {};
   unifi = handleTest ./unifi.nix {};
   unit-php = handleTest ./web-servers/unit-php.nix {};
+  unit-perl = handleTest ./web-servers/unit-perl.nix {};
   upnp.iptables = handleTest ./upnp.nix { useNftables = false; };
   upnp.nftables = handleTest ./upnp.nix { useNftables = true; };
   uptermd = handleTest ./uptermd.nix {};
diff --git a/nixos/tests/web-servers/unit-perl.nix b/nixos/tests/web-servers/unit-perl.nix
new file mode 100644
index 000000000000..e632221747cf
--- /dev/null
+++ b/nixos/tests/web-servers/unit-perl.nix
@@ -0,0 +1,46 @@
+import ../make-test-python.nix (
+  { pkgs, ... }:
+  let
+    testdir = pkgs.writeTextDir "www/app.psgi" ''
+      my $app = sub {
+          return [
+              "200",
+              [ "Content-Type" => "text/plain" ],
+              [ "Hello, Perl on Unit!" ],
+          ];
+      };
+    '';
+
+  in
+  {
+    name = "unit-perl-test";
+    meta.maintainers = with pkgs.lib.maintainers; [ sgo ];
+
+    nodes.machine =
+      {
+        config,
+        lib,
+        pkgs,
+        ...
+      }:
+      {
+        services.unit = {
+          enable = true;
+          config = pkgs.lib.strings.toJSON {
+            listeners."*:8080".application = "perl";
+            applications.perl = {
+              type = "perl";
+              script = "${testdir}/www/app.psgi";
+            };
+          };
+        };
+      };
+    testScript = ''
+      machine.wait_for_unit("unit.service")
+      machine.wait_for_open_port(8080)
+
+      response = machine.succeed("curl -f -vvv -s http://127.0.0.1:8080/")
+      assert "Hello, Perl on Unit!" in response, "Hello world"
+    '';
+  }
+)
diff --git a/pkgs/servers/http/unit/default.nix b/pkgs/servers/http/unit/default.nix
index d9ebb18bc393..255d702caf5e 100644
--- a/pkgs/servers/http/unit/default.nix
+++ b/pkgs/servers/http/unit/default.nix
@@ -68,7 +68,10 @@ in stdenv.mkDerivation rec {
     ${optionalString withRuby_3_2   "./configure ruby   --module=ruby32   --ruby=${ruby_3_2}/bin/ruby"}
   '';
 
-  passthru.tests.unit-php = nixosTests.unit-php;
+  passthru.tests = {
+    unit-perl = nixosTests.unit-perl;
+    unit-php = nixosTests.unit-php;
+  };
 
   meta = with lib; {
     description = "Dynamic web and application server, designed to run applications in multiple languages";