diff --git a/nixos/tests/all-tests.nix b/nixos/tests/all-tests.nix
index 2d44cf924d4f..b4c9fccb23ae 100644
--- a/nixos/tests/all-tests.nix
+++ b/nixos/tests/all-tests.nix
@@ -530,7 +530,6 @@ in {
   networking.networkd = handleTest ./networking.nix { networkd = true; };
   networking.scripted = handleTest ./networking.nix { networkd = false; };
   netbox_3_5 = handleTest ./web-apps/netbox.nix { netbox = pkgs.netbox_3_5; };
-  netbox_3_3 = handleTest ./web-apps/netbox.nix { netbox = pkgs.netbox_3_3; };
   netbox_3_6 = handleTest ./web-apps/netbox.nix { netbox = pkgs.netbox_3_6; };
   netbox-upgrade = handleTest ./web-apps/netbox-upgrade.nix {};
   # TODO: put in networking.nix after the test becomes more complete
diff --git a/pkgs/servers/web-apps/netbox/default.nix b/pkgs/servers/web-apps/netbox/default.nix
index a85f3b1b228d..c90e19380383 100644
--- a/pkgs/servers/web-apps/netbox/default.nix
+++ b/pkgs/servers/web-apps/netbox/default.nix
@@ -1,32 +1,10 @@
-{ lib, nixosTests, callPackage, fetchpatch }:
+{ lib, nixosTests, callPackage, }:
 let
   generic = import ./generic.nix;
 in
 lib.fix (self: {
   netbox = self.netbox_3_6;
 
-  netbox_3_3 = callPackage generic {
-    version = "3.3.10";
-    hash = "sha256-MeOfTU5IxNDoUh7FyvwAQNRC/CE0R6p40WnlF+3RuxA=";
-    extraPatches = [
-      # Allow setting the STATIC_ROOT from within the configuration and setting a custom redis URL
-      ./config_3_3.patch
-      ./graphql-3_2_0.patch
-      # fix compatibility ith django 4.1
-      (fetchpatch {
-        url = "https://github.com/netbox-community/netbox/pull/10341/commits/ce6bf9e5c1bc08edc80f6ea1e55cf1318ae6e14b.patch";
-        sha256 = "sha256-aCPQp6k7Zwga29euASAd+f13hIcZnIUu3RPAzNPqgxc=";
-      })
-    ];
-
-    tests = {
-      netbox = nixosTests.netbox_3_3;
-      inherit (nixosTests) netbox-upgrade;
-    };
-    maintainers = with lib.maintainers; [ n0emis raitobezarius ];
-    eol = true;
-  };
-
   netbox_3_5 = callPackage generic {
     version = "3.5.9";
     hash = "sha256-CJbcuCyTuihDXrObSGyJi2XF+zgWAwcJzjxtkX8pmKs=";
diff --git a/pkgs/top-level/aliases.nix b/pkgs/top-level/aliases.nix
index ecebbf9591ab..80035ceebfd6 100644
--- a/pkgs/top-level/aliases.nix
+++ b/pkgs/top-level/aliases.nix
@@ -1183,6 +1183,7 @@ mapAliases ({
   nccl_cudatoolkit_11 = throw "nccl_cudatoolkit_11 has been renamed to cudaPackages_11.nccl"; # Added 2022-04-04
 
   net_snmp = throw "'net_snmp' has been renamed to/replaced by 'net-snmp'"; # Converted to throw 2023-09-10
+  netbox_3_3 = throw "netbox 3.3 series has been removed as it was EOL"; # Added 2023-09-02
   nagiosPluginsOfficial = monitoring-plugins;
   navit = throw "navit has been removed from nixpkgs, due to being unmaintained"; # Added 2021-06-07
   ncat = throw "'ncat' has been renamed to/replaced by 'nmap'"; # Converted to throw 2022-02-22
diff --git a/pkgs/top-level/all-packages.nix b/pkgs/top-level/all-packages.nix
index f9e256b732c3..9892fa9f70a7 100644
--- a/pkgs/top-level/all-packages.nix
+++ b/pkgs/top-level/all-packages.nix
@@ -11073,7 +11073,7 @@ with pkgs;
   netbootxyz-efi = callPackage ../tools/misc/netbootxyz-efi { };
 
   inherit (callPackage ../servers/web-apps/netbox { })
-    netbox netbox_3_3 netbox_3_5 netbox_3_6;
+    netbox netbox_3_5 netbox_3_6;
 
   netbox2netshot = callPackage ../tools/admin/netbox2netshot { };