diff --git a/tests/functional/flakes/flakes.sh b/tests/functional/flakes/flakes.sh
index 427290883..14529c475 100644
--- a/tests/functional/flakes/flakes.sh
+++ b/tests/functional/flakes/flakes.sh
@@ -93,6 +93,23 @@ foo
 EOF
 chmod +x $nonFlakeDir/shebang-comments.sh
 
+cat > $nonFlakeDir/shebang-different-comments.sh <<EOF
+#! $(type -P env) nix
+# some comments
+// some comments
+* some comments
+\ some comments
+% some comments
+@ some comments
+-- some comments
+(* some comment
+#! nix --offline shell
+#! nix flake1#fooScript
+#! nix --no-write-lock-file --command cat
+foo
+EOF
+chmod +x $nonFlakeDir/shebang-different-comments.sh
+
 cat > $nonFlakeDir/shebang-reject.sh <<EOF
 #! $(type -P env) nix
 # some comments
@@ -607,6 +624,7 @@ expectStderr 1 nix flake metadata "$flake2Dir" --no-allow-dirty --reference-lock
 [[ $($nonFlakeDir/shebang.sh) = "foo" ]]
 [[ $($nonFlakeDir/shebang.sh "bar") = "foo"$'\n'"bar" ]]
 [[ $($nonFlakeDir/shebang-comments.sh ) = "foo" ]]
+[[ "$($nonFlakeDir/shebang-different-comments.sh)" = "$(cat $nonFlakeDir/shebang-different-comments.sh)" ]]
 [[ $($nonFlakeDir/shebang-inline-expr.sh baz) = "foo"$'\n'"baz" ]]
 [[ $($nonFlakeDir/shebang-file.sh baz) = "foo"$'\n'"baz" ]]
 expect 1 $nonFlakeDir/shebang-reject.sh 2>&1 | grepQuiet -F 'error: unsupported unquoted character in nix shebang: *. Use double backticks to escape?'